fluorescence microscopy (Beyotime)
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fluorescence microscopy
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32584 article reviews
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32584 article reviews
fluorescence microscopy - by Bioz Stars,
2026-10
99/100 stars
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Blocking Assay:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Incubation:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Fluorescence:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Targeting cystathionine-β-synthase (CBS)-mediated cell stemness and gut microbiome homeostasis: mechanism and clinical study of combined resveratrol-curcumin treatment for ulcerative colitis. Article Snippet: Background: Durable mucosal healing is the primary therapeutic goal in ulcerative colitis (UC).. Intestinal epithelial stemness and microbiota homeostasis drive this healing process.. Cystathionine-β-synthase (CBS) is a key enzyme in endogenous sulfur metabolism. Article Title: The Role of SERPINE1 in Coordinating Osteogenesis and Angiogenesis of hDPSCs for Bone Regeneration. Article Snippet: Aim: The repair of criticalsized bone defects remains a significant clinical challenge due to the difficulty in achieving spatiotemporal coordination between osteogenesis and angiogenesis.. This study aimed to identify key molecular regulators bridging these processes in human dental pulp stem cells (hDPSCs) and to evaluate the therapeutic efficacy of a bioinspired delivery system comprising SERPINE1loaded hydroxyapatite/chitosan microspheres (HA/CS MS) for vascularised bone regeneration.. Methodology: Temporal transcriptomic profiling of hDPSCs during osteogenic and angiogenic differentiation was employed to screen for candidate dualregulators. Article Title: The response mechanism of mandarin fish (Siniperca chuatsi) astrocyte-like cells to SCRV infection: Insights from Transcriptome Sequencing. Article Snippet: A novel cell line, designated SCAC01 (Siniperca chuatsi Astrocyte clone 01), was established from the brain of mandarin fish (S. chuatsi).. SCAC01 cell line highly expressed the astrocyte-specific marker gfap and could be stably passaged for more than 60 generations.. The cell line exhibited high susceptibility to S. chuatsi rhabdovirus (SCRV) and supported robust viral replication accompanied by obviously cytopathic effects. Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Microscopy:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: The Role of SERPINE1 in Coordinating Osteogenesis and Angiogenesis of hDPSCs for Bone Regeneration. Article Snippet: Aim: The repair of criticalsized bone defects remains a significant clinical challenge due to the difficulty in achieving spatiotemporal coordination between osteogenesis and angiogenesis.. This study aimed to identify key molecular regulators bridging these processes in human dental pulp stem cells (hDPSCs) and to evaluate the therapeutic efficacy of a bioinspired delivery system comprising SERPINE1loaded hydroxyapatite/chitosan microspheres (HA/CS MS) for vascularised bone regeneration.. Methodology: Temporal transcriptomic profiling of hDPSCs during osteogenic and angiogenic differentiation was employed to screen for candidate dualregulators. Article Title: The response mechanism of mandarin fish (Siniperca chuatsi) astrocyte-like cells to SCRV infection: Insights from Transcriptome Sequencing. Article Snippet: A novel cell line, designated SCAC01 (Siniperca chuatsi Astrocyte clone 01), was established from the brain of mandarin fish (S. chuatsi).. SCAC01 cell line highly expressed the astrocyte-specific marker gfap and could be stably passaged for more than 60 generations.. The cell line exhibited high susceptibility to S. chuatsi rhabdovirus (SCRV) and supported robust viral replication accompanied by obviously cytopathic effects. Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Confocal:Article Title: Targeting cystathionine-β-synthase (CBS)-mediated cell stemness and gut microbiome homeostasis: mechanism and clinical study of combined resveratrol-curcumin treatment for ulcerative colitis. Article Snippet: Background: Durable mucosal healing is the primary therapeutic goal in ulcerative colitis (UC).. Intestinal epithelial stemness and microbiota homeostasis drive this healing process.. Cystathionine-β-synthase (CBS) is a key enzyme in endogenous sulfur metabolism. Laser-Scanning Microscopy:Article Title: Targeting cystathionine-β-synthase (CBS)-mediated cell stemness and gut microbiome homeostasis: mechanism and clinical study of combined resveratrol-curcumin treatment for ulcerative colitis. Article Snippet: Background: Durable mucosal healing is the primary therapeutic goal in ulcerative colitis (UC).. Intestinal epithelial stemness and microbiota homeostasis drive this healing process.. Cystathionine-β-synthase (CBS) is a key enzyme in endogenous sulfur metabolism. Staining:Article Title: Empagliflozin-pretreated mesenchymal stem cell-derived extracellular vesicles facilitate cardiac repair via AGGF1 Article Snippet: To verify their uptake ability, EVs were labeled with the red fluorescent dye Dil (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, Beyotime, C1036), and the unbound dye was removed. .. Then, the EVs were co-incubated with H9c2 rat cardiomyocytes, HUVECs, and mouse monocyte - macrophage leukemia cell line RAW 264.7 cells for 24 h. Subsequently, the cytoskeleton was stained with Actin-Tracker Green-488 (Beyotime, C2201S), and the Article Title: The response mechanism of mandarin fish (Siniperca chuatsi) astrocyte-like cells to SCRV infection: Insights from Transcriptome Sequencing. Article Snippet: A novel cell line, designated SCAC01 (Siniperca chuatsi Astrocyte clone 01), was established from the brain of mandarin fish (S. chuatsi).. SCAC01 cell line highly expressed the astrocyte-specific marker gfap and could be stably passaged for more than 60 generations.. The cell line exhibited high susceptibility to S. chuatsi rhabdovirus (SCRV) and supported robust viral replication accompanied by obviously cytopathic effects. Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and |
![Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4487/pmc08114487/pmc08114487__12915_2021_1043_Fig1_HTML.jpg)